ASTM E1191-03a(2014)

1.1 This guide describes procedures for obtaining laboratory data concerning the adverse effects of a test material added to dilution water, but not to food, on certain species of saltwater mysids during continuous exposure from immediately after birth until after the beginning of reproduction using the flow-through technique. These procedures will probably be useful for conducting life-cycle toxicity tests with other species of mysids, although modifications might be necessary.

1.2 Other modifications of these procedures might be justified by special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, results of tests conducted using unusual procedures are not likely to be comparable to results of many other tests. Comparison of results obtained using modified and unmodified versions of these procedures might provide useful information on new concepts and procedures for conducting life-cycle toxicity tests with saltwater mysids.

1.3 These procedures are applicable to all chemicals, either individually or in formulations, commercial products, or known mixtures, that can be measured accurately at the necessary concentrations in water. With appropriate modifications, these procedures can be used to conduct tests on temperature, dissolved oxygen, and pH and on such materials as aqueous effluents (see also Guide E1192), leachates, oils, particulate matter, sediments, and surface waters.

1.4 This guide is arranged as follows:

	Section
Referenced Documents	2
Terminology	3
Summary of Guide	4
Significance and Use	5
Hazards	7
Apparatus	6
Facilities	6.1
Construction Materials	6.2
Metering System	6.3
Test Chambers	6.4
Cleaning	6.5
Acceptability	6.6
Dilution Water	8
Requirements	8.1
Source	8.2
Treatment	8.3
Characterization	8.4
Test Material	9
General	9.1
Stock Solution	9.2
Test Concentration(s)	9.3
Test Organisms	10
Species	10.1
Age	10.2
Source	10.3
Brood Stock	10.4
Food	10.5
Handling	10.6
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