ASTM E1366-11(2016)

1.1 This practice covers procedures for obtaining data concerning toxicity and other effects of a test material to a multi-trophic level freshwater community.

1.2 These procedures also might be useful for studying the fate of test materials and transformation products, although modifications and additional analytical procedures might be necessary.

1.3 Modification of these procedures might be justified by special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, results of tests conducted using unusual procedures are not likely to be comparable to results of many other tests. Comparison of results obtained using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting multi-trophic level tests.

1.4 This practice is arranged as follows:

	Section
Referenced Documents	2
Terminology	3
Summary of Practice	4
Significance and Use	5
Apparatus	6
Facilities	6.1
Container	6.2
Equipment	6.3
Hazards	7
Microcosm Components	8
Medium	8.1
Medium Preparation	8.2
Sediment	8.3
Microcosm Assembly	8.4
Test Material	9
General	9.1
Stock Solution	9.2
Nutrient Control	9.3
Test Concentration(s)	9.4
Test Organisms	10
Algae	10.1
Animals	10.2
Specificity of Organisms	10.3
Sources	10.4
Algal Culture Maintenance	10.5
Animal Culture Maintenance	10.6
	Section
Procedure	11
Experimental Design	11.1
Procedure
Inoculation	11.2
Culling	11.3
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