ASTM E1498-92(1998)

1.1 This guide covers procedures for obtaining laboratory data concerning the adverse effects of a test material added to dilution water on sexual reproduction by seaweeds. The exposure duration is species dependent and is followed by a period of development to allow the evidence of sexual reproduction to appear. There is no exposure to toxicants during the development period. This restricts the tests primarily to the events surrounding egg fertilization, and it minimizes any timelag effects on development that might interfere with correct enumeration of the number of sexual events that occurred. These procedures will probably be useful for conducting sexual reproduction toxicity tests with a variety of species of seaweeds, although modifications might be necessary.

1.2 Other modifications of these procedures might be justified by special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, the results of tests conducted using unusual procedures are not likely to be comparable to those of many other tests. Comparison of the results obtained using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting sexual reproduction tests with seaweeds.

1.3 These procedures are applicable to most chemicals, either individually or in formulations, commercial products, and known mixtures or whole effluents, as well as for use in testing surface waters. With appropriate modifications, these procedures can be used to study the effects of temperature, dissolved oxygen, pH, and such materials as leachates, oils, particulate matter, and sediments.

1.4 The values stated in SI units are to be regarded as the standard.

1.5 This guide is arranged as follows:

Section Referenced Documents 2 Terminology 3 Summary of Guide 4 Significance and Use 5 Apparatus 6 Facilities 6.1 Construction Materials 6.2 Test Chambers 6.3 Cleaning 6.4 Acceptability 6.5 Hazards 7 Dilution and Culture Water 8 Requirements 8.1 Source and Treatment 8.2 Test Material 9 Single Chemicals 9.1 Stock Solutions 9.2 Effluents and Surface Waters 9.3 Test Concentrations 9.4 Test Organism 10 Species 10.1 Life Stage 10.2 Source 10.3 Culture Nutrient Medium 10.4 Procedure 11 Preparation of Plants for a Test 11.1 Test Conditions 11.2 Experimental Design 11.3 Acceptability of Test 12 Calculation of Results 13 Documentation 14 Keywords 15 Appendix X1

1.6 This standard does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. For specific hazards statements, see 6.4 and Section 7.