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ASTM International, 12/01/2012
Publisher: ASTM
File Format: PDF
$34.00$69.00
Published:01/12/2012
Pages:12
File Size:1 file , 230 KB
Note:This product is unavailable in Russia, Ukraine, Belarus
1.1 This guide covers procedures for obtaining laboratory data concerning the adverse effects of a test material added to dilution water on sexual reproduction by seaweeds. The exposure duration is species dependent and is followed by a period of development to allow the evidence of sexual reproduction to appear. There is no exposure to toxicants during the development period. This restricts the tests primarily to the events surrounding egg fertilization, and it minimizes any timelag effects on development that might interfere with correct enumeration of the number of sexual events that occurred. These procedures will probably be useful for conducting sexual reproduction toxicity tests with a variety of species of seaweeds, although modifications might be necessary.
1.2 Other modifications of these procedures might be justified by special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, the results of tests conducted using unusual procedures are not likely to be comparable to those of many other tests. Comparison of the results obtained using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting sexual reproduction tests with seaweeds.
1.3 These procedures are applicable to most chemicals, either individually or in formulations, commercial products, and known mixtures or whole effluents, as well as for use in testing surface waters. With appropriate modifications, these procedures can be used to study the effects of temperature, dissolved oxygen, pH, and such materials as leachates, oils, particulate matter, and sediments.
1.4 The values stated in SI units are to be regarded as the standard.
1.5 This guide is arranged as follows:
Section
Referenced Documents
2
Terminology
3
Summary of Guide
4
Significance and Use
5
Apparatus
6
Facilities
6.1
Construction Materials
6.2
Test Chambers
6.3
Cleaning
6.4
Acceptability
6.5
Hazards
7
Dilution and Culture Water
8
Requirements
8.1
Source and Treatment
8.2
Test Material
9
Single Chemicals
9.1
Stock Solutions
9.2
Effluents and Surface Waters
9.3
Test Concentrations
9.4
Test Organism
10
Species
10.1
Life Stage
10.2
Source
10.3
Culture Nutrient Medium
10.4
Procedure
11
Preparation of Plants for a Test
11.1
Test Conditions
11.2
Experimental Design
11.3
Acceptability of Test
12
Calculation of Results
13
Documentation
14
Keywords
15
Appendix
X1
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. For specific hazards statements, see 6.4 and Section 7.
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