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BS PD ISO/TS 21569-3:2020

Horizontal methods for molecular biomarker analysis. Methods of analysis for the detection of genetically modified organisms and derived products-Construct-specific real-time PCR method for detection of P35S-pat-sequence for screening for genetically modified organisms

BSI Group, 07/08/2020

Publisher: BS

File Format: PDF

$90.00$180.34


Published:08/07/2020

Pages:22

File Size:1 file , 2.3 MB

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This document describes a procedure for the detection of the DNA transition sequence between the 35S promotor (P35S) from Cauliflower mosaic virus and a modified phoshinothricin-acetyltransferase gene (pat) from Streptomyces viridochromogenes. The P35S-pat construct is frequently found in genetically modified plants with tolerance for phosphinothricin-containing herbicides. The P35S-pat construct specific method is based on a real-time PCR and can be used for qualitative and quantitative screening purposes. For identification and quantification of a specific event, a follow-up analysis can be carried out.

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